Western blot analysis of Phospho-C/EBP alpha pThr226 using Phospho-C/EBP alpha pThr226 polyclonal antibody (Product # PA5-36789) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with EGF (0.1ng/ml, 30min), Lane 2: Mouse spleen tissue lysate, Lane 3: Rat spleen tissue lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human C/EBP alpha around the phosphorylation site of Threonine 226|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 30 and 45 kDa.
Purity is >95% by SDS-PAGE.
C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP alpha uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP alpha is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP alpha mRNA and protein, but also the DNA-binding activity of C/EBP alpha. C/EBP alpha functions as an important transcription factor that regulates different genes, including Prolactin gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.