PA1-011 detects phospho-alpha-B crystallin (Ser45) from bovine, human, mouse and rat samples. This antibody does not detect the unphosphorylated form of the protein.
PA1-011 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~20 kDa protein representing recombinant bovine phospho-alpha-B crystallin (Ser45). Immunofluorescence staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining.
The PA1-011 immunogen is a synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin.
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens.
Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha B-crystallin; Alpha(B)-crystallin; Alpha-crystallin B chain; CRYA2; crystallin, alpha 2; crystallin, alpha B; crystallin, alpha polypeptide 2; CTPP2; epididymis secretory protein Li 101; Heat shock protein beta-5; heat shock protein CryAB; heat shock protein HspB5; heat-shock 20 kD like-protein; HSPB5; P23; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Gene Aliases: AACRYA; BOS_14343; CMD1II; Crya-2; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; P23
Molecular Function: chaperone