Peptide Competition and Phosphatase Treatment: Rat brain lysates were either left untreated (1-4) or treated with lambda (l) phosphatase (5). Lysates were then incubated with CaM Kinase II Alpha [pT286] Polyclonal Antibody, Rabbit, following prior incubation.
|Tested species reactivity||Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human CaMKIIa that contains threonine 286. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular response to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD andreduces LTP and learning (Elgersma et al., 2002
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||The Ca2+-calmodulin-dependent kinase II is activated in papillary thyroid carcinoma (PTC) and mediates cell proliferation stimulated by RET/PTC.||Rusciano MR,Salzano M,Monaco S,Sapio MR,Illario M,De Falco V,Santoro M,Campiglia P,Pastore L,Fenzi G,Rossi G,Vitale M||Endocrine-related cancer (17:113)||2010|
|Rat||Not Cited||Pleiotropic effects of cadmium in mesangial cells.||Xiao W,Liu Y,Templeton DM||Toxicology and applied pharmacology (238:315)||2009|
|Rat||Not Cited||Ca(2+)/calmodulin-dependent protein kinase II inhibition by heparin in mesangial cells.||Xiao W,Liu Y,Templeton DM||American journal of physiology. Renal physiology (288:F142)||2005|