Western blot analysis of Phospho-DARPP-32 pSer137 in rat caudal lysate using a Phospho-DARPP-32 pSer137 polyclonal antibody (Product # PA5-35384). Results show a band at ~32kDa. Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen but not by the corresponding dephosphopeptide (not shown).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser137 of rat PPP1R1B conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.1mg/ml BSA, 0.15M NaCl, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, human, mouse and non-human primate based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
Midbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders. One well-studied target for the actions of dopamine is DARPP32. In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons (Ouimet and Greengard, 1990) that also express dopamine D1 receptors (Walaas and Greengard, 1984). The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions (Halpain et al., 1990). Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 (Walaas and Greengard, 1984); phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor (Hemmings et al., 1984). NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32 (Halpain et al., 1990).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.