Left panel: Extracts of Jurkat cells untreated (1) or treated with 10 µM hydrogen peroxide for 10 minutes (2-5) were resolved on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 3% milk-TBST buffer for one hour at room temperature, then incubated with the ETS1[pT38] antibody overnight at 4°C in a 1% milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphothreonine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab') 2 anti-rabbit IgG HRP conjugate (Prod #ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to ETS1[pT38] blocks the signal, verifying the specificity of the antibody. The data also show up-regulation of the signal upon hydrogen peroxide treatment in this cell system. Right panels: Extracts of Jurkat cells untreated (6) or treated with 10 µM hydrogen peroxide for ten minutes (7-10) that were either pretreated with the MEK inhibitor PD98059 (8), the JNK inhibitor SP600125 (9), or the p38 inhibitor SB202190 (10). Western blotting was performed as described above using the ETS1[pT38] antibody or an antibody recognizing total ETS1 protein. The data illustrate regulation of this phosphorylation site by the ERK branch of the MAPK signaling cascade.
|Tested species reactivity||Chicken, Mouse, Rabbit, Rat, Xenopus|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ETS1 that contains threonine 38.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
Ets-1 is a transcription factor involved in the regulation of genes required for extracellular matrix remodeling during metastasis of tumors. It is known to interact with the enhancer of urokinase like plasminogen activator, and promoters of stromelysin-1 and collagenase-1. It is absent from normal gastric mucosa but is expressed in 60% of gastric carcinoma and oral squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activation of MAPK pathways due to DUSP4 loss promotes cancer stem cell-like phenotypes in basal-like breast cancer.
44-1104G was used in western blot to examine how DUSP4 regulates the MAP-ERK kinase and c-jun-NH2-kinase pathways in modifying cancer stem cell-like behavior.
|Balko JM,Schwarz LJ,Bhola NE,Kurupi R,Owens P,Miller TW,Gómez H,Cook RS,Arteaga CL||Cancer research (73:6346)||2013|
Profiling of residual breast cancers after neoadjuvant chemotherapy identifies DUSP4 deficiency as a mechanism of drug resistance.
44-1104G was used in western blot to identify genes associated with neoadjuvant chemotherapy resistance.
|Balko JM,Cook RS,Vaught DB,Kuba MG,Miller TW,Bhola NE,Sanders ME,Granja-Ingram NM,Smith JJ,Meszoely IM,Salter J,Dowsett M,Stemke-Hale K,González-Angulo AM,Mills GB,Pinto JA,Gómez HL,Arteaga CL||Nature medicine (18:1052)||2012|
|Rat||Not Cited||c-Jun N-terminal kinase binding domain-dependent phosphorylation of mitogen-activated protein kinase kinase 4 and mitogen-activated protein kinase kinase 7 and balancing cross-talk between c-Jun N-terminal kinase and extracellular signal-regulated kinase pathways in cortical neurons.||Repici M,Mare L,Colombo A,Ploia C,Sclip A,Bonny C,Nicod P,Salmona M,Borsello T||Neuroscience (159:94)||2009|