Invitrogen

Phospho-ErbB2 (HER-2) (Tyr1248) Monoclonal Antibody (PN2A), Biotin

Product Details

MA5-11364

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 32 publications 32 publications
Immunohistochemistry (IHC)	Assay-dependent	View 13 publications 13 publications
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Neutralization (Neu)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 1 publication 1 publication
ELISA (ELISA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	PN2A
Immunogen	Tyrosine-phosphorylated synthetic peptide corresponding to aa1242-1255 [Lys-TAENPE-pY-LGLDVPV] from the C-terminus of human c-erbB-2 protein (P-Tyr1248) View immunogen
Conjugate	Biotin Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10984779

Product Specific Information

MA5-11364 targets Phospho-HER-2 pTyr1248 in IF, IHC, and WB applications and shows reactivity with Human and Rat samples.

The MA5-11364 immunogen is tyrosine-phosphorylated synthetic peptide corresponding to aa1242-1255 [Lys-TAENPE-pY-LGLDVPV] from the C-terminus of human c-erbB-2 protein (P-Tyr1248).

Target Information

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dual fatty acid synthase and HER2 signaling blockade shows marked antitumor activity against breast cancer models resistant to anti-HER2 drugs.

PloS one

Blancafort A,Giró-Perafita A,Oliveras G,Palomeras S,Turrado C,Campuzano Ò,Carrión-Salip D,Massaguer A,Brugada R,Palafox M,Gómez-Miragaya J,González-Suárez E,Puig T

MA5-11364 was used in western blot to study anti-tumor activity of breast cancer models resistant to anti-HER2 drugs due to a dual fatty acid synthase and HER2 signaling blockade

Thu Apr 28 00:00:00 EDT 2016

Comparison of HER2 and phospho-HER2 expression between biopsy and resected breast cancer specimens using a quantitative assessment method.

PloS one

Bai Y,Cheng H,Bordeaux J,Neumeister V,Kumar S,Rimm DL,Stern DF

MA5-11364 was used in immunohistochemistry and western blot to study HER2 and phospho-HER2 levels in core needle biopsies and in resected breast cancer tissue

Tue Sep 23 00:00:00 EDT 2014

Quantitative measurements of HER2 and phospho-HER2 expression: correlation with pathologic response to neoadjuvant chemotherapy and trastuzumab.

BMC cancer

Cheng H,Bai Y,Sikov W,Sinclair N,Bossuyt V,Abu-Khalaf MM,Harris LN,Rimm DL

MA5-11364 was used in immunohistochemistry to study the predictive value of quantitative measurement of HER2 and phosphorylated HER2 in breast cancer patients receiving neoadjuvant chemotherapy and trastuzumab

Thu May 08 00:00:00 EDT 2014

T47D breast cancer cells switch from ER/HER to HER/c-Src signaling upon acquiring resistance to the antiestrogen fulvestrant.

Cancer letters

Kirkegaard T,Hansen SK,Larsen SL,Reiter BE,Sørensen BS,Lykkesfeldt AE

MA5-11364 was used in western blot to study the switch from ER/HER to HER/c-Src signaling as T47D breast cancer cells acquire fulvestrant resistance

Sat Mar 01 00:00:00 EST 2014

V-ATPase inhibition overcomes trastuzumab resistance in breast cancer.

Molecular oncology

von Schwarzenberg K,Lajtos T,Simon L,Müller R,Vereb G,Vollmar AM

MA5-11364 was used in immunocytochemistry to study the mechanism by which inhibition of the vacuolar ATPase inhibits the growth of trastuzumab-resistant breast cancer

Sat Feb 01 00:00:00 EST 2014

Ganoderma tsugae Extract Inhibits Growth of HER2-Overexpressing Cancer Cells via Modulation of HER2/PI3K/Akt Signaling Pathway.

Evidence-based complementary and alternative medicine : eCAM

Kuo HP,Hsu SC,Ou CC,Li JW,Tseng HH,Chuang TC,Liu JY,Chen SJ,Su MH,Cheng YC,Chou WY,Kao MC

MA5-11364 was used in immunohistochemistry, immunoprecipitation, and western blot to study the role of the HER2-PI3 kinase-Akt pathway in the inhibitory effects of a Gandoderma tsugae extract on the growth of cancer cells overexpressing HER2

Mon May 13 00:00:00 EDT 2013

TIMP-1 overexpression does not affect sensitivity to HER2-targeting drugs in the HER2-gene-amplified SK-BR-3 human breast cancer cell line.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Deng X,Fogh L,Lademann U,Jensen V,Stenvang J,Yang H,Brünner N,Schrohl AS

MA5-11364 was used in western blot to study the lack of effect of TIMP-1 overexpression on HER2 proteolytic shedding and sensitivity to drugs targeting HER2 in a human breast cancer cell line displaying HER2 amplification

Mon Apr 01 00:00:00 EDT 2013

Modulating therapeutic effects of the c-Src inhibitor via oestrogen receptor and human epidermal growth factor receptor 2 in breast cancer cell lines.

European journal of cancer (Oxford, England : 1990)

Fan P,McDaniel RE,Kim HR,Clagett D,Haddad B,Jordan VC

MA5-11364 was used in western blot to study the influence of estrogen receptor and HER2 expression on the therapeutic efficacy of a c-Src inhibitor in a panel of breast cancer cell lines

Sat Dec 01 00:00:00 EST 2012

EGF promotes neuroendocrine-like differentiation of prostate cancer cells in the presence of LY294002 through increased ErbB2 expression independent of the phosphatidylinositol 3-kinase-AKT pathway.

Carcinogenesis

Cortés MA,Cariaga-Martinez AE,Lobo MV,Martín Orozco RM,Motiño O,Rodríguez-Ubreva FJ,Angulo J,López-Ruiz P,Colás B

MA5-11364 was used in western blot to study the mechanism by which EGF promotes neuroendocrine-like differention of prostate cancer cells in the presence of a PI3 kinase inhibitor

Fri Jun 01 00:00:00 EDT 2012

HDAC inhibitor SNDX-275 enhances efficacy of trastuzumab in erbB2-overexpressing breast cancer cells and exhibits potential to overcome trastuzumab resistance.

Cancer letters

Huang X,Wang S,Lee CK,Yang X,Liu B

MA5-11364 was used in western blot to study the effect of a HDAC inhibitor on the efficacy of trastuzumab and its potential to overcome resistance in erbB2-overexpressing breast cancer cells

Mon Aug 01 00:00:00 EDT 2011

Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action.

Breast cancer research : BCR

DeFazio-Eli L,Strommen K,Dao-Pick T,Parry G,Goodman L,Winslow J

MA5-11364 was used in immunoprecipitation to investigate the state of HER1-HER2 interaction and modification in cell lines and tumors

Fri Apr 15 00:00:00 EDT 2011

Lapatinib, a dual HER1/HER2 tyrosine kinase inhibitor, augments basal cleavage of HER2 extracellular domain (ECD) to inhibit HER2-driven cancer cell growth.

Journal of cellular physiology

Vazquez-Martin A,Oliveras-Ferraros C,Cufí S,Del Barco S,Martin-Castillo B,Menendez JA

MA5-11364 was used in western blot to study the mechanisms underlying Lapatinib inhibition of HER2-driven cancer cell growth

Sat Jan 01 00:00:00 EST 2011

Elevated expression of erbB3 confers paclitaxel resistance in erbB2-overexpressing breast cancer cells via upregulation of Survivin.

Oncogene

Wang S,Huang X,Lee CK,Liu B

MA5-11364 was used in western blot to investigate the effect of erbB3 on chemotherapeutic agents against breast cancer

Thu Jul 22 00:00:00 EDT 2010

Presence of HER4 associates with increased sensitivity to Herceptin in patients with metastatic breast cancer.

Breast cancer research : BCR

Sassen A,Diermeier-Daucher S,Sieben M,Ortmann O,Hofstaedter F,Schwarz S,Brockhoff G

MA5-11364 was used in immunohistochemistry to investigate the mechanism for cellular susceptibility to Herceptin

Wed Feb 24 00:00:00 EST 2010

Heterotrimerization of the growth factor receptors erbB2, erbB3, and insulin-like growth factor-i receptor in breast cancer cells resistant to herceptin.

Cancer research

Huang X,Gao L,Wang S,McManaman JL,Thor AD,Yang X,Esteva FJ,Liu B

MA5-11364 was used in western blot to study the role of erbB2, erbB3, and IGF-1R heterodimerization in the resistance of breast cancer cells to herceptin

Mon Feb 01 00:00:00 EST 2010

HDAC inhibitor SNDX-275 induces apoptosis in erbB2-overexpressing breast cancer cells via down-regulation of erbB3 expression.

Cancer research

Huang X,Gao L,Wang S,Lee CK,Ordentlich P,Liu B

MA5-11364 was used in western blot to study the mechanism by which a class I isoform-selective HDAC inhibitor induces apoptosis in erbB2-overexpressing breast cancer cells

Sun Nov 01 00:00:00 EDT 2009

Cancer cells harboring MET gene amplification activate alternative signaling pathways to escape MET inhibition but remain sensitive to Hsp90 inhibitors.

Cell cycle (Georgetown, Tex.)

Wang S,Pashtan I,Tsutsumi S,Xu W,Neckers L

MA5-11364 was used in western blot to study the mechanism by which cancer cells with MET gene amplification can escape MET inhibition

Wed Jul 01 00:00:00 EDT 2009

Incomplete surgical resection of ductal carcinomas in situ results in activation of ERBB2 in residual breast cancer cells.

Endocrine-related cancer

Singer CF,Hudelist G,Fuchs EM,Köstler W,Fink-Retter A,Gschwantler-Kaulich D,Gnant M,Lamm W,Rudas M,Czerwenka K,Kubista E

MA5-11364 was used in immunohistochemistry to study the effect of incomplete cancer resection on ERBB2 activation in residual cancer cells

Sun Mar 01 00:00:00 EST 2009

Merlin regulates transmembrane receptor accumulation and signaling at the plasma membrane in primary mouse Schwann cells and in human schwannomas.

Oncogene

Lallemand D,Manent J,Couvelard A,Watilliaux A,Siena M,Chareyre F,Lampin A,Niwa-Kawakita M,Kalamarides M,Giovannini M

MA5-11364 was used in immunohistochemistry to study the role of merlin on receptor accumulation and signaling at the plasma membrane in normal and tumorigenic Schwann cells

Thu Feb 12 00:00:00 EST 2009

Targeting HER2-positive breast cancer with trastuzumab-DM1, an antibody-cytotoxic drug conjugate.

Cancer research

Lewis Phillips GD,Li G,Dugger DL,Crocker LM,Parsons KL,Mai E,Blättler WA,Lambert JM,Chari RV,Lutz RJ,Wong WL,Jacobson FS,Koeppen H,Schwall RH,Kenkare-Mitra SR,Spencer SD,Sliwkowski MX

MA5-11364 was used in western blot to study the use of an antibody-cytotoxic drug conjugate to target HER2-positive breast cancer

Sat Nov 15 00:00:00 EST 2008

PIK3CA cooperates with other phosphatidylinositol 3'-kinase pathway mutations to effect oncogenic transformation.

Cancer research

Oda K,Okada J,Timmerman L,Rodriguez-Viciana P,Stokoe D,Shoji K,Taketani Y,Kuramoto H,Knight ZA,Shokat KM,McCormick F

MA5-11364 was used in western blot to examine the role of PIK3CA in tumorigenesis

Wed Oct 01 00:00:00 EDT 2008

Additive effects of a prolactin receptor antagonist, G129R, and herceptin on inhibition of HER2-overexpressing breast cancer cells.

Breast cancer research and treatment

Scotti ML,Langenheim JF,Tomblyn S,Springs AE,Chen WY

MA5-11364 was used in western blot to study the additive effects of a prolactin receptor antagonist and herceptin on breast cancer cells overexpressing HER2

Mon Sep 01 00:00:00 EDT 2008

Quantitative characterization of the large-scale association of ErbB1 and ErbB2 by flow cytometric homo-FRET measurements.

Biophysical journal

Szabó A,Horváth G,Szöllosi J,Nagy P

MA5-11364 was used in blocking or activating experiment to develop a method for the quantitative characterization of the association of ErbB1 and ErbB2 by flow cytometric homo-FRET measurements

Fri Aug 01 00:00:00 EDT 2008

Designed TPR modules as novel anticancer agents.

ACS chemical biology

Cortajarena AL,Yi F,Regan L

MA5-11364 was used in western blot to study the anti-tumor effect of novel tetratricopeptide repeat modules targeting Hsp90 function

Thu Mar 20 00:00:00 EDT 2008

Co-targeting insulin-like growth factor I receptor and HER2: dramatic effects of HER2 inhibitors on nonoverexpressing breast cancer.

Cancer research

Chakraborty AK,Liang K,DiGiovanna MP

MA5-11364 was used in western blot to study the anti-proliferative effects of combined inhibition of IGF-1 receptor and HER2 in breast cancer cell lines

Sat Mar 01 00:00:00 EST 2008

Epitope mapping and structural analysis of an anti-ErbB2 antibody A21: Molecular basis for tumor inhibitory mechanism.

Proteins

Hu S,Zhu Z,Li L,Chang L,Li W,Cheng L,Teng M,Liu J

MA5-11364 was used in western blot to study the tumor inhibitory mechanism of an anti-ErbB2 antibody

Fri Feb 15 00:00:00 EST 2008

Overexpression of fatty acid synthase gene activates HER1/HER2 tyrosine kinase receptors in human breast epithelial cells.

Cell proliferation

Vazquez-Martin A,Colomer R,Brunet J,Lupu R,Menendez JA

MA5-11364 was used in western blot to study the role of fatty acid synthase everexpression in activating HER1/HER2 tyrosine kinase receptors in human breast epithelial cells

Fri Feb 01 00:00:00 EST 2008

Hyaluronic acid synthase-1 expression regulates bladder cancer growth, invasion, and angiogenesis through CD44.

Cancer research

Golshani R,Lopez L,Estrella V,Kramer M,Iida N,Lokeshwar VB

MA5-11364 was used in western blot to investigate the effect of hyaluronic acid synthase-1 on bladder cancer development

Tue Jan 15 00:00:00 EST 2008

Nuclear translocation of HER-4/c-erbB-4 is significantly correlated with prognosis of esophageal squamous cell carcinoma.

Journal of surgical oncology

Xu S,Kitayama J,Yamashita H,Souma D,Nagawa H

MA5-11364 was used in immunohistochemistry to study the prognostic significance of nuclear translocation of HER-4/c-erbB-4 in esophageal squamous cell carcinoma

Tue Jan 01 00:00:00 EST 2008

Efficacy and safety of single-agent pertuzumab, a human epidermal receptor dimerization inhibitor, in patients with non small cell lung cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Herbst RS,Davies AM,Natale RB,Dang TP,Schiller JH,Garland LL,Miller VA,Mendelson D,Van den Abbeele AD,Melenevsky Y,de Vries DJ,Eberhard DA,Lyons B,Lutzker SG,Johnson BE

MA5-11364 was used in immunohistochemistry to study the efficacy and safety of a human epidermal receptor dimerization inhibitor in a phase II clinical trial of non-small cell lung cancer

Mon Oct 15 00:00:00 EDT 2007

EGF prevents the neuroendocrine differentiation of LNCaP cells induced by serum deprivation: the modulator role of PI3K/Akt.

Neoplasia (New York, N.Y.)

Martín-Orozco RM,Almaraz-Pro C,Rodríguez-Ubreva FJ,Cortés MA,Ropero S,Colomer R,López-Ruiz P,Colás B

MA5-11364 was used in western blot to investigate the impact of epidermal growth factor on neuroendocrine differentiation of LNCaP cells

Wed Aug 01 00:00:00 EDT 2007

Downregulation of erbB3 abrogates erbB2-mediated tamoxifen resistance in breast cancer cells.

International journal of cancer

Liu B,Ordonez-Ercan D,Fan Z,Edgerton SM,Yang X,Thor AD

MA5-11364 was used in western blot to study the role of erbB3 in erbB2-mediated tamoxifen resistance in breast cancer cells

Tue May 01 00:00:00 EDT 2007

Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer.

European journal of cancer (Oxford, England : 1990)

Vazquez-Martin A,Colomer R,Menendez JA

MA5-11364 was used in immunohistochemistry to investigate the cytokine signature induced by HER2 in breast cancer

Tue May 01 00:00:00 EDT 2007

Phosphorylated HER-2 tyrosine kinase and Her-2/neu gene amplification as predictive factors of response to trastuzumab in patients with HER-2 overexpressing metastatic breast cancer (MBC).

European journal of cancer (Oxford, England : 1990)

Giuliani R,Durbecq V,Di Leo A,Paesmans M,Larsimont D,Leroy JY,Borms M,Vindevoghel A,Jerusalem G,D'Hondt V,Dirix L,Canon JL,Richard V,Cocquyt V,Majois F,Reginster M,Demol J,Kains JP,Delree P,Keppens C,Sotiriou C,Piccart MJ,Cardoso F

MA5-11364 was used in immunohistochemistry to identify prognostic indicators of response/resistance to trastuzumab in metastatic breast cancer

Thu Mar 01 00:00:00 EST 2007

Loss of Hsp90 association up-regulates Src-dependent ErbB2 activity.

Molecular and cellular biology

Xu W,Yuan X,Beebe K,Xiang Z,Neckers L

MA5-11364 was used in western blot to study the effect of loss of HSP90 binding on Src-dependent ErbB2 activity

Mon Jan 01 00:00:00 EST 2007

Hsp90 inhibitor 17-AAG reduces ErbB2 levels and inhibits proliferation of the trastuzumab resistant breast tumor cell line JIMT-1.

Immunology letters

Zsebik B,Citri A,Isola J,Yarden Y,Szöllosi J,Vereb G

MA5-11364 was used in flow cytometry to investigate the influence of 17-AAG on ErbB2 expression and breast cancer cell proliferation

Sat Apr 15 00:00:00 EDT 2006

Constitutive neuregulin-1/ErbB signaling contributes to human vestibular schwannoma proliferation.

Glia

Hansen MR,Roehm PC,Chatterjee P,Green SH

MA5-11364 was used in western blot to study the role of constitutive neuregulin-1/ErbB signaling in human vestibular schwannoma proliferation

Sat Apr 15 00:00:00 EDT 2006

Functional interaction between mouse erbB3 and wild-type rat c-neu in transgenic mouse mammary tumor cells.

Breast cancer research : BCR

Kim A,Liu B,Ordonez-Ercan D,Alvarez KM,Jones LD,McKimmey C,Edgerton SM,Yang X,Thor AD

MA5-11364 was used in western blot to study the interaction between erbB3 and c-neu in transgenic mouse mammary tumor cells

Thu Feb 23 00:00:00 EST 2006

Neuregulin growth factors and their ErbB receptors form a potential signaling network for schwannoma tumorigenesis.

Journal of neuropathology and experimental neurology

Stonecypher MS,Chaudhury AR,Byer SJ,Carroll SL

MA5-11364 was used in immunohistochemistry to study the role of neuregulin growth factors and erbB receptors in Schwannoma tumorigenesis

Wed Feb 01 00:00:00 EST 2006

Vitamin D receptor agonists induce prostatic acid phosphatase to reduce cell growth and HER-2 signaling in LNCaP-derived human prostate cancer cells.

The Journal of steroid biochemistry and molecular biology

Stewart LV,Lyles B,Lin MF,Weigel NL

MA5-11364 was used in western blot to study the effects of vitamin D receptor agonists on androgen-dependent LNCaP human prostate cancer cell growth

Sat Oct 01 00:00:00 EDT 2005

Differential localization of ErbB receptor ensembles influences their signaling in hippocampal neurons.

DNA and cell biology

Chen J,Tseng HC,Dichter MA,Zhang H,Greene MI

MA5-11364 was used in immunocytochemistry to investigate the influence of ErbB localization on its signaling in hippocampal neurons

Thu Sep 01 00:00:00 EDT 2005

Neoadjuvant trastuzumab induces apoptosis in primary breast cancers.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Mohsin SK,Weiss HL,Gutierrez MC,Chamness GC,Schiff R,Digiovanna MP,Wang CX,Hilsenbeck SG,Osborne CK,Allred DC,Elledge R,Chang JC

MA5-11364 was used in immunohistochemistry to study the induction of apoptosis by neoadjuvant trastuzumab in primary breast cancers

Sun Apr 10 00:00:00 EDT 2005

Expression, location, and interactions of ErbB2 and its intramembrane ligand Muc4 (sialomucin complex) in rat mammary gland during pregnancy.

Journal of cellular physiology

Price-Schiavi SA,Andrechek E,Idris N,Li P,Rong M,Zhang J,Carothers Carraway CA,Muller WJ,Carraway KL

MA5-11364 was used in immunohistochemistry and western blot to study the role of ErbB2 and its intramembrane ligand Muc4 in rat mammary gland during pregnancy

Fri Apr 01 00:00:00 EST 2005

A novel CYR61-triggered 'CYR61-alphavbeta3 integrin loop' regulates breast cancer cell survival and chemosensitivity through activation of ERK1/ERK2 MAPK signaling pathway.

Oncogene

Menendez JA,Vellon L,Mehmi I,Teng PK,Griggs DW,Lupu R

MA5-11364 was used in western blot to study the involvement of angiogenic inducer CYR61 in breast cancer cell survival and chemosensitivity

Thu Jan 27 00:00:00 EST 2005

Dietary fatty acids regulate the activation status of Her-2/neu (c-erbB-2) oncogene in breast cancer cells.

Annals of oncology : official journal of the European Society for Medical Oncology

Menendez JA,Ropero S,Lupu R,Colomer R

MA5-11364 was used in western blot to study the role of dietary fatty acids in c-erbB-2 activation in breast cancer cells

Mon Nov 01 00:00:00 EST 2004

Exquisite antitumour response to trastuzumab in a patient with no evidence of Ras-Raf-MAPK and PI3K-Akt pathways activation.

Breast (Edinburgh, Scotland)

Mano MS,Awada A,Minisini A,Durbecq V,Di Leo A,Piccart M

MA5-11364 was used in immunohistochemistry to report on a case of a patient with HER2-overexpressing metastatic breast cancer highly sensitive to trastuzumab treatment

Sun Aug 01 00:00:00 EDT 2004

Synergistic interactions between tamoxifen and trastuzumab (Herceptin).

Clinical cancer research : an official journal of the American Association for Cancer Research

Argiris A,Wang CX,Whalen SG,DiGiovanna MP

MA5-11364 was used in western blot to study the synergistic interactions between tamoxifen and Herceptin in breast cancer

Sun Feb 15 00:00:00 EST 2004

Her-2/neu-triggered intracellular tyrosine kinase activation: in vivo relevance of ligand-independent activation mechanisms and impact upon the efficacy of trastuzumab-based treatment.

British journal of cancer

Hudelist G,Köstler WJ,Attems J,Czerwenka K,Müller R,Manavi M,Steger GG,Kubista E,Zielinski CC,Singer CF

MA5-11364 was used in western blot to study the effect of Her-2/neu proteolytic cleavage and activation on trastuzumab-based treatment

Mon Sep 15 00:00:00 EDT 2003

Profiling receptor tyrosine kinase activation by using Ab microarrays.

Proceedings of the National Academy of Sciences of the United States of America

Nielsen UB,Cardone MH,Sinskey AJ,MacBeath G,Sorger PK

MA5-11364 was used in ELISA to study the receptor tyrosine kinase activation by through antibody microarrays

Tue Aug 05 00:00:00 EDT 2003

High levels of HER-2 expression alter the ability of epidermal growth factor receptor (EGFR) family tyrosine kinase inhibitors to inhibit EGFR phosphorylation in vivo.

Clinical cancer research : an official journal of the American Association for Cancer Research

Christensen JG,Schreck RE,Chan E,Wang X,Yang C,Liu L,Cui J,Sun L,Wei J,Cherrington JM,Mendel DB

MA5-11364 was used in western blot to investigate the influence of HER-2 level on the function of EGFR inhibitors

Sat Dec 01 00:00:00 EST 2001

Tyrphostin AG825 triggers p38 mitogen-activated protein kinase-dependent apoptosis in androgen-independent prostate cancer cells C4 and C4-2.

Cancer research

Murillo H,Schmidt LJ,Tindall DJ

MA5-11364 was used in western blot to study the role of p38 MAP kinase in the induction of apoptosis in androgen-independent prostate cancer cells by the EGFR inhibitor typhostin AG825

Mon Oct 15 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog); c-erb B2/neu protein; C-erbB-2; CD antigen CD340; CD340; epidermal growth factor receptor-related protein; erb-b2; erbb 2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; herstatin; human epidermal growth factor receptor 2; metas; Metastatic lymph node gene 19 protein; MLN 19; NEU proto-oncogene; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; p185neu; phospho ErbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

Gene Aliases: CD340; ERBB2; HER-2; HER-2/neu; HER2; MLN 19; MLN19; NEU; NGL; TKR1

UniProt ID: (Human) P04626

Entrez Gene ID: (Human) 2064, (Rat) 24337

Molecular Function: transmembrane signal receptor

Function(s)

Process(es)

RNA polymerase I core binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding protein C-terminus binding growth factor binding protein phosphatase binding identical protein binding ErbB-3 class receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity protein dimerization activity glycoprotein binding DNA binding ubiquitin protein ligase binding Hsp90 protein binding

Phospho-ErbB2 (HER-2) (Tyr1248) Monoclonal Antibody (PN2A), Biotin

Product Details

MA5-11364

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Neutralization (Neu)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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