Western blot analysis of Phospho-Erythropoietin Receptor pTyr368 in K562 whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-Erythropoietin Receptor pTyr368 polyclonal antibody (Product # PA5-35714).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human Epo-R around the phosphorylation site of Tyrosine 368|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.