Western blot detection of Phospho-FAK pSer910 in extracts of human epithelial carcinoma cells expressing FAK with (lanes 2-5) or without (lane 1) 100 ng/mL Taxol for 18-24 hours in serum-free media to arrest cells in mitosis. Samples were resolved by SDS-PAGE on a 10% Tris-glycine gel, transferred to PVDF and incubated using a Phospho-FAK pSer910 polyclonal antibody (Product # PA1-28423).
|Tested species reactivity||Human, Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the region of FAK that contains serine 910. The sequence is conserved in human, mouse, rat, chicken and frog.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28423 detects FAK from human samples.
PA1-28423 has been successfully used in Western blot applications.
The PA1-28423 immunogen is: Synthetic peptide derived from the region of FAK that contains serine 910. The sequence is conserved in human, mouse, rat, chicken and frog.
Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.