Western blot analysis of Phospho-FAK2 pTyr402 in whole cell extract (60ug per lane) from A431 cells (lane 1) and Raji cells (lane 2) using a Phospho-FAK2 pTyr402 recombinant rabbit monoclonal antibody (Product # 700632) at a dilution of 5ug/ml. Detection was performed using an HRP-conjugated goat anti-rabbit secondary antibody at a dilution of 1:5000 followed by chemiluminescence (ECL). Results show a band at ~115kDa.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide with the sequence ESDI[pY]AEIPDETL corresponding to amino acids 398–410 of Pyk2|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1 ul|
|Western Blot (WB)||4-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
PyK2, also known as protein tyrosine kinase, is known to be expressed in the central nervous system and from cells derived from hematopoietic lineages. In response to various stimuli that increase the intracellular calcium concentration, PyK2 gets rapidly phosphorylated on the tyrosine residues. On activation of the Pyk2 protein, the MAP kinase signaling pathway is activated and its activation brings about modulation of the ion channel function. The phosphorylation of tyrosin is brought about by reactions involving protein tyrosine phosphatases and the RAFT/Pyk2 kinase proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.