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Phospho-FRS2 (Tyr196) Antibody (PA5-105926) in ICC/IF
Immunocytochemistry analysis of Phospho-FRS2 (Tyr196) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-FRS2 (Tyr196) polyclonal antibody (Product # PA5-105926) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat ... View More
Product Details
PA5-105926
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human FRS2(Accession Q8WU20), corresponding to amino acid residues around phosphorylated Tyr196.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2817325
Product Specific Information
Antibody detects endogenous levels of FRS2 only when phosphorylated at Tyr196.
Target Information
Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGFR signalling adaptor; FGFR substrate 2; FGFR-signaling adaptor SNT; Fibroblast growth factor receptor substrate 2; FRS2-alpha; SNT-1; SNT2; Suc1-associated neurotrophic factor target 1
Gene Aliases: 4732458E18; C330018A15Rik; FRS1A; FRS2; FRS2A; FRS2alpha; SNT; SNT-1; SNT1
UniProt ID: (Human) Q8WU20, (Mouse) Q8C180
Entrez Gene ID: (Human) 10818, (Mouse) 327826, (Rat) 314850
transmembrane receptor protein tyrosine kinase adaptor activity
Ras guanyl-nucleotide exchange factor activity
fibroblast growth factor receptor binding
insulin receptor binding
neurotrophin TRKA receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
phosphatase activator activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
activation of MAPK activity
gastrulation with mouth forming second
organ induction
lens development in camera-type eye
ventricular septum development
transmembrane receptor protein tyrosine kinase signaling pathway
neuroblast proliferation
fibroblast growth factor receptor signaling pathway
anterior/posterior axis specification, embryo
positive regulation of signal transduction
forebrain development
regulation of apoptotic process
optic placode formation involved in camera-type eye formation
regulation of epithelial cell proliferation
prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis
lens fiber cell development
regulation of ERK1 and ERK2 cascade
MAPK cascade
activation of MAPKK activity
transmembrane receptor protein tyrosine phosphatase signaling pathway
G-protein coupled receptor signaling pathway
regulation of phosphatidylinositol 3-kinase signaling
phosphatidylinositol-3-phosphate biosynthetic process
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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