Immunofluorescent analysis of Phospho-GSK-3beta pSer9 in GSK-3alpha (-/-) mouse embryonic fibroblasts (MEFs), FGF-treated, using a Phospho-GSK-3beta pSer9 monoclonal antibody (Product # MA5-14873) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues around pSer9 of human GSK-3ß|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lithium treatment decreases activities of tau kinases in a murine model of senescence.
MA5-14873 was used in western blot to study the effect of lithium on the acctivity of tau kinases in a mouse model of senescence
|Tajes M,Gutierrez-Cuesta J,Folch J,Ferrer I,Caballero B,Smith MA,Casadesus G,Camins A,Pallás M||Journal of neuropathology and experimental neurology (67:612)||2008|