|Tested species reactivity||Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 of rat GRIA1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Western Blot (WB)||1: 1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
This antibody is predicted to react with human, mouse and non-human primate based on 100% sequence homology.
In Western blot, this antibody detects a ~100 kDa protein representing phospho-GluR1 (pSer845) in rat brain lysate. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of 4 distinct subunits (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser845. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cocaine-induced behavioral sensitization decreases the expression of endocannabinoid signaling-related proteins in the mouse hippocampus.
OPA1-04118 was used in western blot to determine if the hippocampal gene/protein expression of relevant glutamate signaling components, cannabinoid type 1 receptor, and endocannabinoid metabolic enzymes were altered following acute and/or repeated cocaine administration
|Blanco E,Galeano P,Palomino A,Pavón FJ,Rivera P,Serrano A,Alen F,Rubio L,Vargas A,Castilla-Ortega E,Decara J,Bilbao A,de Fonseca FR,Suárez J||European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology (26:477)||2016|
Attenuation of cocaine-induced conditioned locomotion is associated with altered expression of hippocampal glutamate receptors in mice lacking LPA1 receptors.
OPA1-04118 was used in western blot to investigate the contribution of LPA1 receptors to drug abuse
|Blanco E,Bilbao A,Luque-Rojas MJ,Palomino A,Bermúdez-Silva FJ,Suárez J,Santín LJ,Estivill-Torrús G,Gutiérrez A,Campos-Sandoval JA,Alonso-Carrión FJ,Márquez J,de Fonseca FR||Psychopharmacology (220:27)||2012|