Immunofluorescent analysis of Phospho-Histone H3 pSer28 in mouse anterior prostate using a Phospho-Histone H3 pSer28 monoclonal antibody (Product # MA1-24751) at a dilution of 1:2000 (red) and a phospho-S6 (pSer235/236) antibody (green). Nuclei were stained using DAPI (blue).
|Tested species reactivity||Bovine, Hamster, Mouse, Human|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 23-35 of human Histone H3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24751 detects Histone H3 (phospho S28) from human, mouse, bovine, and hamster samples.
MA1-24751 has been successfully used in immunocytochemistry, flow cytometry and Western blot procedures. Western blot detects a band at ~15 kDa. A suggested positive control is whole extract of cultured human acute T cell leukemia Jurdat cells, treated with Nocodazole.
The MA1-24751 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 23-35 of human Histone H3.
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Histone H3.3 is a nuclear protein and a replication-independent member of the histone H3 family that has a role in nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3.3 constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. It has a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Expression of H3F3A is common throughout the cell cycle independent of DNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.