Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated insulin receptor substrate-1 (IRS-1). The final product is generated by affinity chromatography using IRS-1 derived peptide that is phosphorylated at serine 616.
The antibody has been used in western blotting. Previous lots of this antibody have been used in immunohistochemistry. In western analysis the positive control used was human embryonic kidney cell line transfected with a vector encoding IRS-1 (293T) and stimulated with a phorbol ester, TPA.
Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis, and insulin signal transduction. Insulin Receptor Substrate-1 (IRS-1) is a 165 kDa multi-phosphorylated cytoplasmic docking protein involved in metabolic and proliferative signaling by insulin, IL-4, and other cytokines. IRS-1 functions to enhance growth hormone-induced proliferative signaling. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. Activation of protein kinase C (PKC) stimulates a kinase which phosphorylates IRS-1 at serine 616, resulting in inhibition of insulin signaling in the cell, possibly serving as a potential mechanism for insulin resistance in some forms of obesity. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option. To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Insulin receptor substrate 1; Insulin receptor substrate-1; IRS-1; IRS1; pp185
Gene Aliases: G972R; HIRS-1; IRS-1; IRS1; IRS1IRM