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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human integrin alpha 4 that contains serine 988.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28431 detects Integrin alpha 4 from human samples.
PA1-28431 has been successfully used in immunoprecipitation and Western blot applications. The predicted molecular weight by Western blot is 150 kDa.
The PA1-28431 immunogen is antiserum raised against a chemically synthesized phosphopeptide derived from a region of human integrin alpha 4 that contains serine 988.
Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohnis disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery. Phosphorylation of the serine 988 residue on the integrin alpha 4 cytoplasmic tail inhibits its interaction with the focal adhesion complex associated protein paxillin, and modulates its effects on cell motility and spreading.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
269C wild type; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; integrin alpha 4; integrin alpha-4; integrin alpha-4 subunit; integrin alpha-IV; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha