|Flow Cytometry (Flow)||1:20|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Western Blot (WB)||1:500-1:2000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from a region of human MEK1 that contains serine 218 and serine 222|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
MAP2K1 is a dual specificity mitogen-activated protein kinase (MAPK) kinase. It is an essential component of the MAP kinase pathway and stimulates the enzymatic activity of MAP kinases in response to various signals. Growth factors, cytokines, and proto-oncogenes transduce their signals through the activation of RAS, which leads to activation of the serine/threonine kinase RAF, and then to activation of MAPK kinase. MAP2K1 is activated by the Raf family member p74raf-1, through phosphorylation of serine residues at positions 217 and 221 in the activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; EC 184.108.40.206; ERK activator kinase 1; ERK activator kinase 2; kinase MEK1; MAP kinase kinase 1; MAP kinase kinase 2; MAP kinase/Erk kinase; MAP kinase/Erk kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MAPKK1; MEK 1; MEK 2; mitogen activated protein kinase kinase 1; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 2, p45; MP2K1; PRKMK1; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen activated, kinase 2, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Aliases: AA589381; CFC3; CFC4; MAP2K1; MAP2K2; MAPKK1; MAPKK2; MEK1; MEK2; MEKK1; MK2; MKK1; MKK2; PRKMK1; PRKMK2
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