Western blot detection of Phospho-MEK7/MAP2K7 pSer271 + pThr275 in extracts prepared from background extracts with GST-tagged fusion protein expressing MEK7. Samples were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight and probed using a Phospho-MEK7/MAP2K7 pSer271 + pThr275 polyclonal antibody (Product # PA1-28418).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (Human) derived from a region of human MEK 7 that contains serine 271 and threonine 275. The sequence is conserved in mouse.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28418 detects MEK7 from human samples.
PA1-28418 has been successfully used in Western blot applications.
The PA1-28418 immunogen is: Synthetic peptide (Human) derived from a region of human MEK 7 that contains serine 271 and threonine 275. The sequence is conserved in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.