Western blot analysis of Phospho-Mcl-1 pSer159/Thr163 in extracts from HeLa cells transfected with human Mcl-1, untreated or treated with lambda-phosphatase, using Phospho-Mcl-1 pSer159/Thr163 polyclonal antibody (Product # PA5-17125) (upper) or a Mcl-1 polyclonal antibody (lower).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding human pSer159/Thr163|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.