Western blot analysis of Phospho-NMDA NR2B Subunit pSer1480 in rat hippocampal lysate using a polyclonal antibody (Product # PA1-4733). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser1480 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
|Tested species reactivity||Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 of rat GRIN2B conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on 100% sequence homology.
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activated CaMKII couples GluN2B and casein kinase 2 to control synaptic NMDA receptors.
PA1-4733 was used in western blot to study the control of synaptic NMDA receptors by CaMKII, GluN2B and CK2
|Sanz-Clemente A,Gray JA,Ogilvie KA,Nicoll RA,Roche KW||Cell reports (3:607)||2013|