Western blot analysis of Phospho-NMDA Receptor 2B pSer1166 in rat hippocampal lysate using a Phospho-NMDA Receptor 2B pSer1166 polyclonal antibody (Product # PA5-35401). Results show a band at ~180kDa. Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (lane 2), but not by the corresponding dephospho-peptide (not shown).
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1166 of rat GRIN2B conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 50% glycerol, 0.1mg/ml BSA, 0.15M NaCl|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with canine, non-human primate and Xenopus based on 100% sequence homology.
The NMDA Receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS. The NMDAR is also potentiated by protein phosphorylation. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of th NMDAR2 (NR2 A-D) subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.