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|Tested species reactivity||Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within the NR2B subunit of the NMDA receptor, which includes phosphorylated Tyr1472.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.01% BSA, 50% glycerol|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-86558 specifically recognizes the 180 kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor when phosphorylated at Tyr1472. Predicted to react with bovine, chicken, dog, human, monkey, mouse and zebrafish based on sequence homology.
For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM sodium orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. In Western blot, this antibody detects a band at ~180 kDa in rat brain hippocampal lysate. In Western blot, this antibody also labels proteins of 65kDa and 115kDa.
Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue Homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dendritic function of tau mediates amyloid-beta toxicity in Alzheimer's disease mouse models.
PA1-86558 was used in western blot to investigate the function of tau in neuronal dendrites for A-beta toxicity
|Ittner LM,Ke YD,Delerue F,Bi M,Gladbach A,van Eersel J,Wölfing H,Chieng BC,Christie MJ,Napier IA,Eckert A,Staufenbiel M,Hardeman E,Götz J||Cell (142:387)||2010|
glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor ionotropic, NMDA 2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B