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Dot blot analysis of Phospho-NR4A1 pSer351 polyclonal antibody (PA5-12771) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S351 of human NR4A1|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse and rat based on sequence homology.
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
early response protein NAK1; GFRP1; growth factor-inducible nuclear protein N10; HMR; hormone receptor; NAK1; nerve growth factor IB nuclear receptor variant 1; NR4A1; nuclear hormone receptor NUR/77; nuclear receptor subfamily 4, group A, member 1; orphan nuclear receptor HMR; Orphan nuclear receptor NR4A1; orphan nuclear receptor TR3; ST-59; steroid receptor TR3; testicular receptor 3; TR3 orphan receptor
GFRP1; HMR; N10; NAK-1; NAK1; NGFIB; NP10; NR4A1; NUR77; TR3