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PAK1/2/3 [pT423] phosphospecific antibody. A549 cells plated on fibronectin, serum starved overnight then stimulated with serum for 1 hour. Green = PAK [pT243], red = actin, blue = Hoechst 33342. Similar staining patterns were seen with PAK [pS141] (data not shown).
|Tested species reactivity||Human|
|Published species reactivity||Bovine, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PAK that contains threonine 423 (for PAK1). The sequence is conserved across many species for all three main isoforms of PAK.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|p21-activated kinase regulates endothelial permeability through modulation of contractility.||Stockton RA,Schaefer E,Schwartz MA||The Journal of biological chemistry (279:46621)||2004|
2; 3; adriamycin resistance-associated; alpha-PAK; C-t-PAK2; gamma-PAK; oligophrenin-3; OPHN3; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 2; p21-Activated Protein Kinase-1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p58; p65-PAK; PAK-1; PAK-2; PAK-2p27; PAK-2p34; PAK-3; Pak1; Pak2; Pak3; S6/H4 kinase; STE20 homolog, yeast
ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK1; PAK2; PAK3; PAK3beta; PAK65; PAKalpha; PAKgamma