PAK1/2/3 [pT423] phosphospecific antibody. A549 cells plated on fibronectin, serum starved overnight then stimulated with serum for 1 hour. Green = PAK [pT243], red = actin, blue = Hoechst 33342. Similar staining patterns were seen with PAK [pS141] (data not shown).
|Tested species reactivity||Human|
|Published species reactivity||Bovine, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PAK that contains threonine 423 (for PAK1). The sequence is conserved across many species for all three main isoforms of PAK.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
p21-Activated kinase (PAK) regulates cytoskeletal reorganization and directional migration in human neutrophils.
44-942G was used in immunocytochemistry to elucidate how Rho GTPases orchestrate cytoskeletal dynamics in migrating neutrophils.
|Itakura A,Aslan JE,Kusanto BT,Phillips KG,Porter JE,Newton PK,Nan X,Insall RH,Chernoff J,McCarty OJ||PloS one (8:null)||2013|
|p21-activated kinase regulates endothelial permeability through modulation of contractility.||Stockton RA,Schaefer E,Schwartz MA||The Journal of biological chemistry (279:46621)||2004|