Western blot analysis of pancreatic acinar (AR42J) cells using Phospho-PERK pThr980 Monoclonal Antibody (Product # MA5-15033). Membranes containing whole cell extracts from untreated (-) AR42J cells or AR42J cells treated (+) for 20 minutes with thapsigargin (Tg) were probed with MA5-15033 followed by an anti-rabbit IgG secondary antibody.
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pThr980 of mouse PERK|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
HCV induces transforming growth factor ß1 through activation of endoplasmic reticulum stress and the unfolded protein response.
MA5-15033 was used in western blot to determine the induction of transforming growth factor beta1 through activation of endoplasmic reticulum stress and the unfolded protein response due to HCV
|Chusri P,Kumthip K,Hong J,Zhu C,Duan X,Jilg N,Fusco DN,Brisac C,Schaefer EA,Cai D,Peng LF,Maneekarn N,Lin W,Chung RT||Scientific reports (6:null)||2016|
Protein tyrosine phosphatase receptor R and Z1 expression as independent prognostic indicators in oral squamous cell carcinoma.
MA5-15033 was used in western blot to study the function of PTPRR and PTPRZ1 proteins in oral cavity squamous cell carcinoma
|Du¿-Szachniewicz K,Wo¿niak M,Nelke K,Gamian E,Gerber H,Zió¿kowski P||Head and neck (37:1816)||2015|
Legionella suppresses the host unfolded protein response via multiple mechanisms.
MA5-15033 was used in western blot to examine the role of the host unfolded protein response during L. pneumophila infection
|Treacy-Abarca S,Mukherjee S||Nature communications (6:null)||2015|