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Western blot analysis of pancreatic acinar (AR42J) cells using Phospho-PERK pThr980 Monoclonal Antibody (Product # MA5-15033). Membranes containing whole cell extracts from untreated (-) AR42J cells or AR42J cells treated (+) for 20 minutes with thapsigargin (Tg) were probed with MA5-15033 followed by an anti-rabbit IgG secondary antibody.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pThr980 of mouse PERK|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
eukaryotic translation initiation factor 2 alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; heme-regulated EIF2-alpha kinase; hsPEK; OTTHUMP00000207187; OTTHUMP00000207188; pancreatic EIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase
AI427929; Eif2ak3; PEK; Perk