|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human PKD1 around the phosphorylation site of Serine 910|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 115 kDa.
Purity is >95% by SDS-PAGE.
Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction. Protein kinase D (PKD), also called PKCu, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway. In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCu. Phosphorylation at Ser916 correlates with PKD/PKCu catalytic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: nPKC-D1; nPKC-mu; PKC mu; PKC u; PKC-u; PKCmu; PKCu; PKD; PKD1; PRKCM; PRKD1; Protein kinase C mu type; protein kinase C, mu; Protein kinase D; Serine/threonine-protein kinase D1
Gene Aliases: nPKC-D1; nPKC-mu; PKC-MU; PKCM; PKD; PKD1; PRKCM; PRKD1
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