Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (Product # PA1-820) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-820 detects phospho-peroxisome proliferator activated receptor (PPAR) alpha S12 from mouse tissues.
PA1-820 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to phospho-PPAR alpha S12 from mouse adipose tissue extract.
The PA1-820 immunogen is a synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha. This peptide (Cat. # PEP-181) is available for use in neutralization and control experiments.
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR and quote;s). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR and quote;s are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR and quote;s can induce transcription of acyl coenzyme A oxidase and cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AS601245, an Anti-Inflammatory JNK Inhibitor, and Clofibrate Have a Synergistic Effect in Inducing Cell Responses and in Affecting the Gene Expression Profile in CaCo-2 Colon Cancer Cells.
PA1-820 was used in western blot to study the synergistic effects of a JNK kinase inhibitor and the PPARgamma ligand clofibrate on the gene expression profile of CaCo-2 colon cancer cells
|Cerbone A,Toaldo C,Pizzimenti S,Pettazzoni P,Dianzani C,Minelli R,Ciamporcero E,Roma G,Dianzani MU,Canaparo R,Ferretti C,Barrera G||PPAR research (2012:null)||2012|
Dysregulation of the peroxisome proliferator-activated receptor target genes by XPD mutations.
PA1-820 was used in western blot to study the relationship between adipose tissue's hypoplasia in XP-D patients and PPARs' faliure in transactivation
|Compe E,Drané P,Laurent C,Diderich K,Braun C,Hoeijmakers JH,Egly JM||Molecular and cellular biology (25:6065)||2005|
MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response.
PA1-820 was used in EMSA assay to characterize MCF-7 and T47D human breast cancer cells in terms of peroxisomal response
|Kilgore MW,Tate PL,Rai S,Sengoku E,Price TM||Molecular and cellular endocrinology (129:229)||1997|