Immunofluorescent analysis of Phospho-Progesterone Receptor (green) in T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with Phospho-Progesterone Receptor pSer190 mouse monoclonal antibody (Product # 37-8200), at a dilution of 1:100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 superclonal goat anti-mouse IgG secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic phospho peptide derived from a region of the human Progesterone Receptor (hPR) protein surrounding the phosphorylated Ser190 residue.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/ml BSA|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
37-8200 detects Phospho-Progesterone Receptor in Human samples.
37-8200 has been successfully used in western blot, immunofluorescence and immunohistochemistry in human samples. Phospho-Progesterone Receptor (Ser 190) antibody detects both progesterone receptor A and B forms when phosphorylated at Ser26 and Ser190, respectively.
The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.