Immunofluorescent analysis of Phospho-Progesterone Receptor pSer554 showing staining in the nucleus of NT2D1 cells. NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a Phospho-Progesterone Receptor pSer554 polyclonal antibody (Product # PA5-31313) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 549 and 561 of Human Progesterone Receptor|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31313 targets Phospho-Progesterone Receptor pSer554 in IHC (P), IP, and WB applications and shows reactivity with Human samples.
The PA5-31313 immunogen is synthetic peptide corresponding to a region within amino acids 549 and 561 of Human Progesterone Receptor.
This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform A has not been clearly determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.