Western blot analysis of Phospho-RelB pSer552 using Phospho-RelB pSer552 polyclonal antibody (Product # PA5-36837) at a dilution of 1:500. Lane 1: Hela cell lysate treated with TNF-alpha, Lane 2: Mouse stomach tissue lysate, Lane 3: Mouse colon tissuel lysate, Lane 4: Rat colon tissue lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human Rel B around the phosphorylation site of Serine 552|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 70 kDa.
Purity is >95% by SDS-PAGE.
The NFkB transcription factor was originally identified as a protein complex consisting of a DNA-binding subunit and an associated protein. The DNAbinding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A second protein designated p52 (previously referred to as p49) has been identified that can act as an alternative NFkB subunit. Rel B does not bind with high affinity to NFkB sites, but heterodimers between Rel B and p50 bind with an affinity comparable to that of p50 NFkB homodimers. However, Rel B/p50 heterodimers, in contrast to NFkB heterodimers, transactivates transcription of promotors containing kB binding sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.