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Immunofluorescence analysis of STAT1 [pS727] was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT1 [pS727] Rabbit polyclonal Antibody (44382G) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Mouse , Human|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human STAT1 that contains serine 727. The sequence is conserved in mouse.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||10µl|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
STAT1 (also known as signal transducers and activators of transcription) is a member of the STAT family of transcription factors. STAT proteins undergo phosphorylation in response to cytokines and growth factors and are translocated to the nucleus. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. The protein is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. The STAT1 gene is located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
RIPK1 ensures intestinal homeostasis by protecting the epithelium against apoptosis.
44-382G was used in western blot to elucidate the role of RIPK1 using conditional knockout mice
|Takahashi N,Vereecke L,Bertrand MJ,Duprez L,Berger SB,Divert T,Gonçalves A,Sze M,Gilbert B,Kourula S,Goossens V,Lefebvre S,Günther C,Becker C,Bertin J,Gough PJ,Declercq W,van Loo G,Vandenabeele P||Nature (513:95)||2014|
IL-4 and IL-13 negatively regulate TNF-alpha- and IFN-gamma-induced beta-defensin expression through STAT-6, suppressor of cytokine signaling (SOCS)-1, and SOCS-3.
||Albanesi C,Fairchild HR,Madonna S,Scarponi C,De Pità O,Leung DY,Howell MD||Journal of immunology (Baltimore, Md. : 1950) (179:984)||2007|
Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line.
||Kaszubska W,Falls HD,Schaefer VG,Haasch D,Frost L,Hessler P,Kroeger PE,White DW,Jirousek MR,Trevillyan JM||Molecular and cellular endocrinology (195:109)||2002|
2010005J02Rik, CANDF7, STAT91, AA408197, ISGF-3
signal transducer and activator of transcription 1, signal transducer and activator of transcription 1-alpha/beta, signal transducer and activator of transcription-1, transcription factor ISGF-3 components p91/p84, Signal transducer and activator of transcription 1-alpha/beta, STAT1