Western blot analysis using an FG pancreatic carcinoma cell line stably expressing vector (FG-V) with the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Cells were incubated overnight at 4°C with 1% BSA/TBST and probed with a Phospho-Src pTyr215 polyclonal antibody (Product # PA5-12697) at a dilution of 1:500.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y215 of human SRC|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chicken, mouse, rat and Xenopus and zebrafish based on sequence homology.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Both isoforms 1 and 2 encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.