|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospeptide corresponding to an amino acid sequence within TPH2, which includes phosphorylated Ser19.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.01% BSA, 50% glycerol|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Predicted to react with bovine, mouse and zebrafish based on sequence homology.
For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM sodium orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. In Western blot, this antibody detects a band at ~55 kDa on lysates from human dorsal Raphe nucleus.
Tryptophan Hydroxylase (TPH) is a rate-limiting enzyme which catalyses the 5-hydroxylation of tryptophan, to form the amino acid 5-hydroxytryptophan (5-HTP), in the first stage of the biosynthesis of the neurotransmitter serotonin (5-hydroxytryptamine/5HT). Mammalian TPH is encoded by two genes: TPH1 expressed in peripheral tissues e.g. skin and gut, and TPH2 primarily expressed in the brain. Studies have demonstrated the regulation/enhancement of TPH activity through phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaMKII), the latter of which phosphorylates a Ser19 site within the regulatory domain of TPH2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.