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Invitrogen
Phospho-VE-cadherin (Tyr658) Polyclonal Antibody
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FIGURE: 1 / 15
Phospho-VE-cadherin (Tyr658) Antibody (44-1144G) in WB
Extracts of HUVEC cells either untreated (1) or treated (2-5)with 100 ng/mL VEGF for 10. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the VE-cadherin (pY658) (Product # 44-1144G).
Product Details
44-1144G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Bovine,
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VE-cadherin that contains tyrosine 658. This sequence is conserved in chicken, chimpanzee, cow, dog, mouse, pig, and rat.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VE-cadherin. The final product is generated by affinity chromatography using an VE-cadherin-derived peptide that is phosphorylated at tyrosine 658.
Positive controls used with this antibody in western blotting were HUVEC +/- VEGF.
Target Information
VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 7B4 antigen; 7B4/cadherin-5; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; unnamed protein product; Vascular endothelial cadherin; VE-cadherin
Gene Aliases: 7B4; AA408225; CD144; CDH5; VE-Cad; Vec; VEcad; VECD
UniProt ID: (Human) P33151, (Mouse) P55284
Entrez Gene ID: (Human) 1003, (Mouse) 12562
receptor binding
calcium ion binding
protein binding
beta-catenin binding
protein phosphatase binding
receptor signaling complex scaffold activity
vascular endothelial growth factor receptor 2 binding
ion channel binding
cadherin binding
metal ion binding
fibrinogen binding
BMP receptor binding
protein binding involved in cell-cell adhesion
protein tyrosine kinase binding
cadherin
cell morphogenesis
endothelial cell morphogenesis
blood vessel maturation
cell migration involved in sprouting angiogenesis
cellular calcium ion homeostasis
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
cell surface receptor signaling pathway
transforming growth factor beta receptor signaling pathway
negative regulation of cell proliferation
positive regulation of gene expression
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
positive regulation of cell migration
positive regulation of BMP signaling pathway
negative regulation of microtubule polymerization
positive regulation of protein complex assembly
adherens junction organization
maintenance of blood-brain barrier
regulation of vascular permeability
blood vessel endothelial cell migration
cell-cell adhesion mediated by cadherin
positive regulation of angiogenesis
negative regulation of inflammatory response
bicellular tight junction assembly
cell-cell adhesion
protein localization to bicellular tight junction
positive regulation of intracellular signal transduction
positive regulation of establishment of endothelial barrier
regulation of establishment of cell polarity
negative regulation of endothelial cell apoptotic process
single organismal cell-cell adhesion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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