Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Vav1 protein immunoprecipitated from K562 cell extracts was resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL Vav1 [pY160] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase (Prod # ALI4405) and signals were detected using the Tropix WesternStar™ method. The data show that only the peptide corresponding to Vav1 [pY160] blocks the antibody signal, thereby demonstrating the specificity of the antibody.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A chemically synthesized phosphopeptide derived from a region of human Vav1 that contains tyrosine 160|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||Molecular dissection of 2B4 signaling: implications for signal transduction by SLAM-related receptors.||Chen R,Relouzat F,Roncagalli R,Aoukaty A,Tan R,Latour S,Veillette A||Molecular and cellular biology (24:5144)||2004|
VAV; vav 1 guanine nucleotide exchange factor; vav 1 oncogene; Vav proto-oncogene