Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Phospho-Zap-70 (Tyr319, Tyr352) Monoclonal Antibody (GT1686)
FIGURE: 1 / 4
Phospho-Zap-70 (Tyr319, Tyr352) Antibody (MA5-35926) in ICC/IF
ZAP70 (phospho Tyr319)/Syk (phospho Tyr352) antibody [GT1686] detects ZAP70 (phospho Tyr319)/Syk (phospho Tyr352) protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZAP70 (phospho Tyr319)/Syk (phospho Tyr352) stained by ZAP70 (phospho Tyr319)/Syk (phospho Tyr352) antibody [GT1686] (Product # MA5-35926) diluted at 1:500. Blue: F... View More
Product Details
MA5-35926
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT1686
Immunogen
Carrier-protein conjugated synthetic peptide corresponding to residues around human ZAP70 (phospho Tyr319)/Syk (phospho Tyr352). The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2866543
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; kinase ZAP70; Selective T cell defect; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; ZA70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Gene Aliases: ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70; ZAP70
UniProt ID: (Human) P43403
Entrez Gene ID: (Human) 7535
adaptive immune response
macrophage activation involved in immune response
neutrophil activation involved in immune response
protein phosphorylation
inflammatory response
immune response
transmembrane receptor protein tyrosine kinase signaling pathway
peptidyl-tyrosine phosphorylation
T cell differentiation
positive regulation of cell adhesion mediated by integrin
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
T cell activation
B cell activation
positive regulation of mast cell degranulation
beta selection
positive thymic T cell selection
negative thymic T cell selection
innate immune response
positive regulation of B cell differentiation
positive regulation of T cell differentiation
positive regulation of alpha-beta T cell differentiation
positive regulation of alpha-beta T cell proliferation
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
B cell receptor signaling pathway
T cell aggregation
T cell migration
regulation of platelet aggregation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support