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Immunofluorescent analysis of Phospho-MAPK1/3 pThr202/Tyr204 (green) in HeLa cells either left untreated (left panel) or treated with 50nM PMA (right panel) for 10 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-MAPK1/3 pThr202/Tyr204 polyclonal antibody (Product # 36-8800) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human , Rat|
|Published species reactivity||Rat , Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to the region surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1+2 (p44/42 MAP kinase).|
|Storage buffer||0.01M HEPES, pH 7.5, with 100µg/ml BSA, 50% glycerol, 0.15M NaCl|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with Xenopus and zebrafish based on 100% sequence homology.
36-8800 is specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.
Extracellular signal-regulated kinase 2 (Erk2) is also known as mitogen activated protein kinase 2, microtubule-associated protein-2 kinase MAP kinase 2, p42-MAPK or EGF receptor T669 (ERT1). Mek2 activates Erk2 by phosphorylation of neighbouring threonine-183 and tyrosine-185 residues. Erk2, a serine-threonine kinase phosphorylates microtubule associated protein-2 and myelin basic protein. This kinase is an important proximal componant of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The role of multicellular aggregation in the survival of ErbB2-positive breast cancer cells during extracellular matrix detachment.
36-8800 was used in western blot to study the mechanism for the metastasis of ErbB2-positive breast cancer cells.
|Rayavarapu RR,Heiden B,Pagani N,Shaw MM,Shuff S,Zhang S,Schafer ZT||The Journal of biological chemistry (290:8722)||2015|
The important roles of RET, VEGFR2 and the RAF/MEK/ERK pathway in cancer treatment with sorafenib.
||Mao WF,Shao MH,Gao PT,Ma J,Li HJ,Li GL,Han BH,Yuan CG||Acta pharmacologica Sinica (33:1311)||2012|
Post-receptor IGF1 insensitivity restricted to the MAPK pathway in a Silver-Russell syndrome patient with hypomethylation at the imprinting control region on chromosome 11.
||Montenegro LR,Leal AC,Coutinho DC,Valassi HP,Nishi MY,Arnhold IJ,Mendonca BB,Jorge AA||European journal of endocrinology / European Federation of Endocrine Societies (166:543)||2012|
Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction.
||Jang IK,Cronshaw DG,Xie LK,Fang G,Zhang J,Oh H,Fu YX,Gu H,Zou Y||Proceedings of the National Academy of Sciences of the United States of America (108:7926)||2011|
15-Deoxy-Delta12,14-prostaglandin J2 regulates endogenous Cot MAPK kinase kinase 1 activity induced by lipopolysaccharide.
||Caivano M,Rodriguez C,Cohen P,Alemany S||The Journal of biological chemistry (278:52124)||2003|
Antihypertensive drug Valsartan promotes dendritic spine density by altering AMPA receptor trafficking.
36-8800 was used in immunohistochemistry to elucidate the molecular mechanism by which Valsartan regulates cognitive function.
|Sohn YI,Lee NJ,Chung A,Saavedra JM,Scott Turner R,Pak DT,Hoe HS||Biochemical and biophysical research communications (439:464)||2013|
Osteopontin is linked to p65 and MMP-9 expression in pulmonary adenocarcinoma but not in malignant pleural mesothelioma.
||Frey AB,Wali A,Pass H,Lonardo F||Histopathology (50:720)||2007|
A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis.
36-8800 was used in immunocytochemistry to study the effect of a novel amyloid-binding small molecule on Ras-mediated spinogenesis.
|Megill A,Lee T,DiBattista AM,Song JM,Spitzer MH,Rubinshtein M,Habib LK,Capule CC,Mayer M,Turner RS,Kirkwood A,Yang J,Pak DT,Lee HK,Hoe HS||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:9306)||2013|
ERK1, p41mapk, Erk2, ERK2, p44mapk, p40, p41, p44-MAPK, p44, P42MAPK, Esrk1, PRKM3, PRKM2, PRKM1, p44erk1, HS44KDAP, ERK, Prkm3, ERT1, ERT2, MNK1, MAPK2, MAPK1, P44MAPK, HUMKER1A, Erk-1, P44ERK1, p38, p44-ERK1, ERK-1
ERK-2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 2, extracellular signal-regulated kinase 2, mitogen-activated protein kinase 1, mitogen-activated protein kinase 2, p42-MAPK, protein tyrosine kinase ERK2, MAP kinase isoform p44, MAPK 1, extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, insulin-stimulated MAP2 kinase, microtubule-associated protein 2 kinase, mitogen-activated protein kinase 3, ERT1, extracellular-signal-regulated kinase 2, MAP kinase 3, MAPK 3, mitogen-activated 3, Mitogen-activated protein kinase 1, MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 3, ERK-1, p44-MAPK, MAPK3, ERK1, PRKM3, p44