Western blot detection of Phospho-p44 MAPK + p42 MAPK pThr202 + pTyr204 + pTyr187 in extracts prepared from PC12 cells left unstimulated (lane 1) or stimulated with sorbitol (lanes 2-6). Samples were resolved by SDS-PAGE on a 10% polyacrylamide gel, transferred to PVDF, and probed using a Phospho-p44 MAPK + p42 MAPK pThr202 + pTyr204 + pTyr187 polyclonal antibody (Product # PA1-26678).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human ERK 1 + 2 containing threonine 202/185 and tyrosine 204/187. This portion is conserved am|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26678 detects ERK1 + ERK2 (phospho T185 + T202) from human samples. PA1-26678 is expected to cross react with a wide range of species due to sequence homology.
PA1-26678 has been successfully used in Western blot procedures.
The PA1-26678 immunogen is a synthetic phosphopeptide corresponding to a portion of human ERK 1 + 2 containing threonine 202/185 and tyrosine 204/187. This portion is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit
kinase involved in intracellular signaling; component of Mapk signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.