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Western blot detection of Phospho-p44 MAPK + p42 MAPK pThr202 + pTyr204 + pTyr187 in extracts prepared from PC12 cells left unstimulated (lane 1) or stimulated with sorbitol (lanes 2-6). Samples were resolved by SDS-PAGE on a 10% polyacrylamide gel, transferred to PVDF, and probed using a Phospho-p44 MAPK + p42 MAPK pThr202 + pTyr204 + pTyr187 polyclonal antibody (Product # PA1-26678).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human ERK 1 + 2 containing threonine 202/185 and tyrosine 204/187. This portion is conserved am|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26678 detects ERK1 + ERK2 (phospho T185 + T202) from human samples. PA1-26678 is expected to cross react with a wide range of species due to sequence homology.
PA1-26678 has been successfully used in Western blot procedures.
The PA1-26678 immunogen is a synthetic phosphopeptide corresponding to a portion of human ERK 1 + 2 containing threonine 202/185 and tyrosine 204/187. This portion is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit
kinase involved in intracellular signaling; component of Mapk signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ERK-1; ERK-2; ERK1; ERK2; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK3; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 2; p42-MAPK; p44-MAPK; PRKM1; PRKM2; PRKM3; protein tyrosine kinase ERK2
ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; HS44KDAP; HUMKER1A; MAPK1; MAPK2; MAPK3; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3