Immunohistochemical analysis of a mouse lung transduced with a human placental alkaline phosphatase (hPLAP) expression vector and stained with Placental Alkaline Phosphatase Polyclonal Antibody (PA1-29125). Paraffin-embedded lung sections were protease-treated for 12 minutes and then stained with PA1-29125 (1:100), followed by an alkaline phosphatase-conjugated secondary antibody and colorimetric substrate (red). Tissues were counterstained with hematoxylin (blue). Data courtesy of the Innovators Program.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified PLAP from human placenta|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29125 detects Placental alkaline phosphatase from human samples. Does not react with canine, mouse, rat, and feline samples.
PA1-29125 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), immunoprecipitation, and Western blot applications.
The PA1-29125 immunogen is purified PLAP from human placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.