Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A549 (Lane 2), HEK-293 (Lane 3), SK-OV-3 (Lane 4) and COLO 205 (Lane 5). The blots were probed with Mouse Anti-PKP3 Monoclonal Antibody (Product # 35-7600, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 87 kDa band corresponding to PKP3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide from Armadillo domain of human Plakophilin-3.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in apunctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of ~87 kD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MMP7 is required to mediate cell invasion and tumor formation upon Plakophilin3 loss.
35-7600 was used in western blot to investigate the role of MMP7 in cell invasion and tumor formation
|Basu S,Thorat R,Dalal SN||PloS one (10:null)||2015|
Plakophilin3 loss leads to an increase in PRL3 levels promoting K8 dephosphorylation, which is required for transformation and metastasis.
35-7600 was used in western blot to investigate the effects of K8 filament stabilization
|Khapare N,Kundu ST,Sehgal L,Sawant M,Priya R,Gosavi P,Gupta N,Alam H,Karkhanis M,Naik N,Vaidya MM,Dalal SN||PloS one (7:null)||2012|
|Human||Not Cited||Plakophilin 3 oncogene as prognostic marker and therapeutic target for lung cancer.||Furukawa C,Daigo Y,Ishikawa N,Kato T,Ito T,Tsuchiya E,Sone S,Nakamura Y||Cancer research (65:7102)||2005|
Unimpaired skin carcinogenesis in Desmoglein 3 knockout mice.
35-7600 was used in immunocytochemistry to explore the role of desmosomal cadherins in carcinogenesis.
|Baron S,Hoang A,Vogel H,Attardi LD||PloS one (7:null)||2012|
E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly.
35-7600 was used in immunocytochemistry and western blot to test if plakophilin3 is required for desmosome formation
|Gosavi P,Kundu ST,Khapare N,Sehgal L,Karkhanis MS,Dalal SN||Cellular and molecular life sciences : CMLS (68:1439)||2011|
|Not Applicable||Not Cited||
The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.
35-7600 was used in ChIP assay, immunocytochemistry, immunohistochemistry, and western blot to show that PKP3 is transcriptionally repressed by ZEB1 in metastatic cancer cells
|Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A||FEBS letters (581:1617)||2007|
||Plakophilin 3 oncogene as prognostic marker and therapeutic target for lung cancer.||Furukawa C,Daigo Y,Ishikawa N,Kato T,Ito T,Tsuchiya E,Sone S,Nakamura Y||Cancer research (65:7102)||2005|