|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ORF Human podoplanin ectodomain expressed in E.coli.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||0.75-2.0 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is normal lung.
Human Podoplanin (gp36) is a 36kDa type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver.
Podoplanin is absent on blood endothelium and therefore MA1-82747 is readily able to distinguish between lymphatic vessels and blood vasculature. Although the specific function of this protein is undetermined, Podoplanin is reported to be a reliable indicator of tumour lymphangiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.