Immunohistochemical staining of formalin-fixed, paraffin-embedded section of Creutzfeldt-Jakob Disease cerebral cortex tissue (Immunohistochemical staining of prion plaque) using a CD230/Prion protein PrP polyclonal antibody (Product # PA1-84495) at 1:200 dilution.
|Tested species reactivity||Bovine, Hamster, Human, Sheep|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 79-97 of the N-terminus of human prion protein|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for immunohistochemical applications is CJD brain tissue. This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. Alternative splicing results in multiple transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.