|Tested species reactivity||Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal sequence of human prolactin receptor intermediate isoform.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Re-evaluation of the prolactin receptor expression in human breast cancer.||Galsgaard ED,Rasmussen BB,Folkesson CG,Rasmussen LM,Berchtold MW,Christensen L,Panina S||The Journal of endocrinology (201:115)||2009|
|Human||Not Cited||Re-evaluation of the prolactin receptor expression in human breast cancer.||Galsgaard ED,Rasmussen BB,Folkesson CG,Rasmussen LM,Berchtold MW,Christensen L,Panina S||The Journal of endocrinology (201:115)||2009|
|Not Applicable||Not Cited||
SRC family kinases accelerate prolactin receptor internalization, modulating trafficking and signaling in breast cancer cells.
34-4800 was used in western blot to demonstrate that src family kinases mediates ligand-initiated long isoform of the PRL receptor internalization, down-regulation, and signal transduction in breast cancer cells
|Piazza TM,Lu JC,Carver KC,Schuler LA||Molecular endocrinology (Baltimore, Md.) (23:202)||2009|
Endogenous human prolactin and not exogenous human prolactin induces estrogen receptor alpha and prolactin receptor expression and increases estrogen responsiveness in breast cancer cells.
34-4800 was used in western blot to study endogenous prolactin
|Gutzman JH,Miller KK,Schuler LA||The Journal of steroid biochemistry and molecular biology (88:69)||2004|
Inhibition of prolactin (PRL)-induced proliferative signals in breast cancer cells by a molecular mimic of phosphorylated PRL, S179D-PRL.
34-4800 was used in western blot to test if a mimic of phosphorylated human prolactin, S79D-PRL, initiates signaling in mammary tumor cells alone and in combination with unmodified prolactin.
|Schroeder MD,Brockman JL,Walker AM,Schuler LA||Endocrinology (144:5300)||2003|
|Human||1:167||Identification of functional prolactin (PRL) receptor gene expression: PRL inhibits lipoprotein lipase activity in human white adipose tissue.||Ling C,Svensson L,Odén B,Weijdegård B,Edén B,Edén S,Billig H||The Journal of clinical endocrinology and metabolism (88:1804)||2003|