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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human recombinant proteasome 19S subunit S2.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-964 detects proteasome 19S subunit S2 from human cells.
PA1-964 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 100 kDa protein representing proteasome 19S subunit S2 in HeLa cell lysate. This antibody detects, to a lesser extent an ~50-kDa protein which could correspond to subunit degradation product.
PA1-964 antigen is recombinant human proteasome 19S subunit S2.
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman"e;s syndrome, and Liddle syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
PA1-964 was used in western blot to use a proteomics approch to identify the range of PARKIN substrates during mitochondrial depolarization
|Sarraf SA,Raman M,Guarani-Pereira V,Sowa ME,Huttlin EL,Gygi SP,Harper JW||Nature (496:372)||2013|
Proteasomal non-catalytic subunit PSMD2 as a potential therapeutic target in association with various clinicopathologic features in lung adenocarcinomas.
PA1-964 was used in western blot to investigate the effect of PSMD2 knockdown in different lung cancer cell lines
|Matsuyama Y,Suzuki M,Arima C,Huang QM,Tomida S,Takeuchi T,Sugiyama R,Itoh Y,Yatabe Y,Goto H,Takahashi T||Molecular carcinogenesis (50:301)||2011|
55.11 protein; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; protein 55.11; TNFR-associated protein 2; tumor necrosis factor type 1 receptor-associated protein 2
P97; PSMD2; RPN1; S2; TRAP2