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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:200-1:4000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:150 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Dog, Human, Mouse, Rat |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI1A8 |
Immunogen |
Full length human recombinant protein of human RFC4 produced in HEK293T cell. |
Conjugate |
Unconjugated |
Form |
lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 8% trehalose |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Human Replication Factor C (RFC) is a hetero-pentameric protein consisting of the p140, p40, p38, p37 and p36 subunits. RFC functions in the loading of the DNA polymerase delta processivity factor (PCNA, Proliferating Cell nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus 1, Rad 1 and Rad 9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: A1 37 kDa subunit; Activator 1 37 kDa subunit; Activator 1 subunit 4; replication factor C (activator 1) 4, 37kDa; Replication factor C 37 kDa subunit; Replication factor C subunit 4; RF-C 37 kDa subunit; RFC 37 kDa subunit; RFC p37
Gene Aliases: A1; AI894123; AU040575; RFC37; RFC4
UniProt ID: (Human) P35249, (Mouse) Q99J62
Entrez Gene ID: (Human) 5984, (Dog) 478667, (Mouse) 106344, (Rat) 288003
Molecular Function:
DNA-directed DNA polymerase
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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