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|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugated.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||1:500 - 1:2000|
|Immunofluorescence (IF)||1:500 - 1:2000|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000 - 1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15257 recognizes native and denatured forms of RFP (Red Fluorescent Protein) and its many variants: tag-RFP, turbo-RFP, DsRed, mCherry, mOrange, etc.
MA5-15257 has successfully been used in immunoprecipitation, ELISA, Western blotting, immunohistochemistry, immunocytochemistry and immunofluorescence applications.
MA5-15257 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles."
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
LIG4 mediates Wnt signalling-induced radioresistance.
MA5-15257 was used in immunohistochemistry to learn mediation of Wnt signaling-induced radioresistance by LIG4
|Jun S,Jung YS,Suh HN,Wang W,Kim MJ,Oh YS,Lien EM,Shen X,Matsumoto Y,McCrea PD,Li L,Chen J,Park JI||Nature communications (7:null)||2016|
TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity.
MA5-15257 was used in immunohistochemistry to report that osmotic stress regulates the activation of immunity in neonatal zebrafish
|Galindo-Villegas J,Montalban-Arques A,Liarte S,de Oliveira S,Pardo-Pastor C,Rubio-Moscardo F,Meseguer J,Valverde MA,Mulero V||Journal of immunology (Baltimore, Md. : 1950) (196:738)||2016|