|Tested species reactivity||Human, Mouse, Yeast|
|Published species reactivity||Yeast|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Replication protein A purified from U293 cells.|
|Storage buffer||PBS with 0.1mg/ml BSA|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||2-3 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2-3 µg/ml|
|Immunoprecipitation (IP)||1 µg|
|Western Blot (WB)||4-6 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ChIP assay (ChIP)||See 2 publications below|
MA1-25889 detects RPA34 from human, mouse and yeast samples.
MA1-25889 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and western blot procedures.
The MA1-25889 immunogen is replication protein A purified from U293 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dpb11 protein helps control assembly of the Cdc45·Mcm2-7·GINS replication fork helicase.
MA1-25889 was used in ChIP assay to study Dpb11 binding in S phase
|Dhingra N,Bruck I,Smith S,Ning B,Kaplan DL||The Journal of biological chemistry (290:7586)||2015|
Cdc45 protein-single-stranded DNA interaction is important for stalling the helicase during replication stress.
MA1-25889 was used in ChIP assay to study the role of the Cdc45 interaction with single-stranded DNA in stalling the Mcm2-7 helicase during replicative stress
|Bruck I,Kaplan DL||The Journal of biological chemistry (288:7550)||2013|