Western blot analysis was performed on whole cell extracts (30 ug lysate) of HeLa (Lane 1) and PC-3 (Lane 2). The blots were probed with ABfinity TM Anti-PRDX6 Recombinant Rabbit Monoclonal Antibody (Product# 702211, 2 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # 656120) at dilutions 1:3,000 (Fig. 1), 1:5,000 (Fig. 2) and 1:10,000 (Fig. 3). A 25 kDa band corresponding to PRDX6 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.4, with 4mg/ml BSA, 40% glycerol|
|Contains||0.1% Proclin 300|
|Storage Conditions||4° C|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:3,000-1:4,000|
|Western Blot (WB)||1:3,000 - 1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Electronic control of gene expression and cell behaviour in Escherichia coli through redox signalling.
65-6120 was used in western blot to develop an electrogenetic device that uses redox biomolecules to control transcription from a simple synthetic gene circuit in bacteria
|Tschirhart T,Kim E,McKay R,Ueda H,Wu HC,Pottash AE,Zargar A,Negrete A,Shiloach J,Payne GF,Bentley WE||Nature communications (8:null)||2017|
|Not Applicable||Not Cited||
TRIM28 is an Epigenetic Barrier to Induced Pluripotent Stem Cell Reprogramming.
65-6120 was used in western blot to characterize TRIM28 as an epigenetic barrier to generate reprogramming of pluripotent stem cells
|Miles DC,de Vries NA,Gisler S,Lieftink C,Akhtar W,Gogola E,Pawlitzky I,Hulsman D,Tanger E,Koppens M,Beijersbergen RL,van Lohuizen M||Stem cells (Dayton, Ohio) (35:147)||2017|
|Not Applicable||Not Cited||
Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74.
65-6120 was used in western blot to study multimodal inhibition of HIV-1 by PF74 and the roles of capsid-interacting host factors
|Saito A,Ferhadian D,Sowd GA,Serrao E,Shi J,Halambage UD,Teng S,Soto J,Siddiqui MA,Engelman AN,Aiken C,Yamashita M||Journal of virology (90:5808)||2016|
Macrophage migration inhibitory factor is a possible candidate for the induction of microalbuminuria in diabetic db/db mice.
65-6120 was used in western blot to identify genes involved in diabetic microalbuminuria
|Watanabe T,Tomioka NH,Doshi M,Watanabe S,Tsuchiya M,Hosoyamada M||Biological and pharmaceutical bulletin (36:741)||2013|
Streptococcus pneumoniae requires iron for its viability and expresses two membrane proteins that bind haemoglobin and haem.
65-6120 was used in western blot to study iron acquisition by Streptococcus pneumoniae
|Romero-Espejel ME,González-López MA,Olivares-Trejo Jde J||Metallomics : integrated biometal science (5:384)||2013|
Activities of ceftobiprole and other cephalosporins against extracellular and intracellular (THP-1 macrophages and keratinocytes) forms of methicillin-susceptible and methicillin-resistant Staphylococcus aureus.
65-6120 was used in western blot to measure the intracellular activity of ceftobiprole against a panel of hospital-acquired and community-acquired methicillin-resistant S. aureus strains
|Lemaire S,Glupczynski Y,Duval V,Joris B,Tulkens PM,Van Bambeke F||Antimicrobial agents and chemotherapy (53:2289)||2009|
Thiazolidinediones inhibit REG Ialpha gene transcription in gastrointestinal cancer cells.
65-6120 was used in western blot to report that PPARgamma-agonist thiazolidinediones suppress cell proliferation and REG Ialpha protein/mRNA expression in gastrointestinal cancer cells
|Yamauchi A,Takahashi I,Takasawa S,Nata K,Noguchi N,Ikeda T,Yoshikawa T,Shervani NJ,Suzuki I,Uruno A,Unno M,Okamoto H,Sugawara A||Biochemical and biophysical research communications (379:743)||2009|
Exercise training acts as a therapeutic strategy for reduction of the pathogenic phenotypes for Alzheimer's disease in an NSE/APPsw-transgenic model.
65-6120 was used in western blot to test if exercise ameliorates the molecular pathogenic phenotypes in the brains of transgenic mice with Alzheimer's disease
|Um HS,Kang EB,Leem YH,Cho IH,Yang CH,Chae KR,Hwang DY,Cho JY||International journal of molecular medicine (22:529)||2008|
Epidermal growth factor receptor-dependent and -independent pathways in hydrogen peroxide-induced mitogen-activated protein kinase activation in cardiomyocytes and heart fibroblasts.
65-6120 was used in western blot to investigate the role of the epidermal growth factor receptor in H2O2-induced MAPK activation using cardiac cells
|Purdom S,Chen QM||The Journal of pharmacology and experimental therapeutics (312:1179)||2005|
Ciliary neurotrophic factor inhibits differentiation of photoreceptor-like cells in rat pineal glands in vitro.
65-6120 was used in western blot to elucidate the role of ciliary neurotrophic factor in mammalian development.
|Hata K,Araki M,Yamamori T||Brain research. Developmental brain research (143:179)||2003|
|Not Applicable||Not Cited||Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.||Clutter MR,Heffner GC,Krutzik PO,Sachen KL,Nolan GP||Cytometry. Part A : the journal of the International Society for Analytical Cytology (77:1020)||2010|
|Not Applicable||Not Cited||
Expression of sperm protein 22 (SP22) in the rat ovary during different reproductive states.
65-6120 was used in immunohistochemistry - paraffin section to assess the presence of sperm protein 22 transcripts in the rat ovary
|Benoit AM,LaVoie HA,McCoy GL,Blake CA||Experimental biology and medicine (Maywood, N.J.) (232:910)||2007|
Expression of CCAAT/enhancer binding proteins alpha and beta in the porcine ovary and regulation in primary cultures of granulosa cells.
65-6120 was used in immunohistochemistry - paraffin section study CCAAT/enhancer binding proteins alpha and beta in the porcine ovary during the estrous cycle
|Gillio-Meina C,Hui YY,LaVoie HA||Biology of reproduction (72:1194)||2005|
Expression of the GATA-4 and GATA-6 transcription factors in the fetal rat gonad and in the ovary during postnatal development and pregnancy.
65-6120 was used in immunohistochemistry - paraffin section to study the distribution of GATA-4 and GATA-6 in rat fetal gonad and the postnatal ovary during development and pregnancy
|Lavoie HA,McCoy GL,Blake CA||Molecular and cellular endocrinology (227:31)||2004|
Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developing Drosophila eye.
65-6120 was used in immunohistochemistry to test if Egfr regulates Atonal restriction
|Rodrigues AB,Werner E,Moses K||Development (Cambridge, England) (132:4697)||2005|
Tumour burden and interleukin-2 dose affect the interaction between low-dose total body irradiation and interleukin 2.
65-6120 was used in immunohistochemistry to optimize the combination therapy of low-dose total body irradiation and interleukin 2 to treat metastatic malignant melanoma
|Safwat A,Aggerholm N,Roitt I,Overgaard J,Hokland M||European journal of cancer (Oxford, England : 1990) (40:1412)||2004|
IgE ELISA using antisera derived from epsilon chain antigenic peptides detects allergen-specific IgE in allergic horses.
65-6120 was used in ELISA to develop and characterize antibodies to assess equine allergy
|Kalina WV,Pettigrew HD,Gershwin LJ||Veterinary immunology and immunopathology (92:137)||2003|