Immunofluorescent analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody (Product # MA1-013) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant full-length human Rap1|
|Storage buffer||PBS with 30% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of MA1-013 detects an ~21kDa protein.
Rap1 is part of the Ras family of GTPases and is encoded by Rap1a and Rap1b. Rap1 cycles between two conformational states depending on whether GDP bound (active form) or GTP bound (inactive form) and primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion and tumor invasion. Rap1 may also regulate proliferation, differentiation and survival through multiple downstream effectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.