Immunofluorescence analysis of Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/ml Rat primary antibody for 3 hours at room temperature. Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate (Product # SA510020) was used at a concentration of 4µg/ml in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Rat IgG-heavy and light chain|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against bovine, chicken, human, mouse, rabbit and sheep|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:125|
|Immunocytochemistry (ICC)||4 µg/ml|
|Immunofluorescence (IF)||4 µg/ml|
|Immunohistochemistry (IHC)||1:50 - 1:2,000|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:5,000 - 1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, human, mouse, rabbit and sheep.
Thermo Scientific Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Wnts are expressed in the spinal cord of adult mice and are differentially induced after injury.
SA5-10020 was used in immunohistochemistry to study the expression of Wnt family members in the adult murine spinal cord following injury
|González-Fernández C,Fernández-Martos CM,Shields SD,Arenas E,Javier Rodríguez F||Journal of neurotrauma (31:565)||2014|