Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.
Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of mouse splenocytes and human peripheral blood cells. This should be used at the same concentration as the experimental antibody.
Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser
Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2